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Found 4 matching solutions for this experiment
Upstream tips |
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Add the MTT Reagent to each well at a 1:10 ratio.
Incubate the wells 2-4 hours or overnight at 37°C.
Add Detergent Solution and incubate in the dark for 2-4 hours at room temperature |
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Protocol tips |
Add the MTT Reagent to each well at a 1:10 ratio.
Incubate the wells 2-4 hours or overnight at 37°C.
Add Detergent Solution and incubate in the dark for 2-4 hours at room temperature |
Upstream tips |
Protocol tips |
Downstream tips |
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Stain cells with 0.5 ml staining solution containing 0.5 μl Live-Dye (1 mM) and 0.5 μl PI (2.5 mg/ml).
Incubate for 15 min at 37°C |
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Protocol tips |
Stain cells with 0.5 ml staining solution containing 0.5 μl Live-Dye (1 mM) and 0.5 μl PI (2.5 mg/ml).
Incubate for 15 min at 37°C |
Upstream tips |
Protocol tips |
Downstream tips |
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Add ethidium-calcein mixture, and incubate for 30 min in a 37 °C incubato |
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Protocol tips |
Add ethidium-calcein mixture, and incubate for 30 min in a 37 °C incubato |
Upstream tips |
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Downstream tips |
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Add working solution and incubate for 15–30 min at 37 °C |
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Protocol tips |
Add working solution and incubate for 15–30 min at 37 °C |
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