Live / Dead assay yeast - Clostridium sporogenes

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Biotium

Upstream tips	Protocol tips	Downstream tips
	Add 0.7 mL isopropyl alcohol into the tube with 0.3 mL of 0.85% NaCl and mix well (final concentration of isopropyl alcohol: 70%) for preparing dead bacteria. Incubate both samples at room temperature for 1 hour, mixing every 15 minutes

Protocol tips
Add 0.7 mL isopropyl alcohol into the tube with 0.3 mL of 0.85% NaCl and mix well (final concentration of isopropyl alcohol: 70%) for preparing dead bacteria. Incubate both samples at room temperature for 1 hour, mixing every 15 minutes

Manufacturer protocol Publication protocol 1 Relevant paper

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