Mammalian cell culture media A549

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Gibco™ RPMI 1640 Medium

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 5% heat-inactivated fetal bovine serum (Hyclone, South Logan, UT, USA), 2 mm glutamine and antibiotics (100 U ml−1 penicillin and 100 μg ml−1 streptomycin)

Protocol tips
supplemented with 5% heat-inactivated fetal bovine serum (Hyclone, South Logan, UT, USA), 2 mm glutamine and antibiotics (100 U ml−1 penicillin and 100 μg ml−1 streptomycin)

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL)

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL)

Manufacturer protocol Publication protocol 1 Relevant paper

HyClone™ Dulbecco's Modified Eagles Medium

Cytiva

Upstream tips	Protocol tips	Downstream tips
	DMEM containing 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin

Protocol tips
DMEM containing 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin

Manufacturer protocol Publication protocol 1 Relevant paper

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