Mammalian cell culture media BPAEC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 2 matching solutions for this experiment

Medium 231

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
Following Ad pre-treatment, cells were exposed to normoxia (5% CO2 – 95% air) or hyperoxia (5% CO2 – 95% O2) in presence or absence of Ad for the desired lengths of time (3–24 h).	supplement the media with 10% FBS, non-essential amino acids, antibiotics, and endothelial growth factor.

Upstream tips
Following Ad pre-treatment, cells were exposed to normoxia (5% CO2 – 95% air) or hyperoxia (5% CO2 – 95% O2) in presence or absence of Ad for the desired lengths of time (3–24 h).
Protocol tips
supplement the media with 10% FBS, non-essential amino acids, antibiotics, and endothelial growth factor.

Manufacturer protocol Publication protocol 1 Relevant paper

Nutrient Mixture F-12 Ham

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	supplement the media with 10% FBS and antibiotics

Protocol tips
supplement the media with 10% FBS and antibiotics

Manufacturer protocol Publication protocol 1 Relevant paper

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