Mammalian cell culture media DH82

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Minimum Essential Medium (EMEM), Quality Biological

VWR

Upstream tips	Protocol tips	Downstream tips
	Eagle's Minimum Essential Medium (EMEM, Quality Biological Inc., # 112-018-101) containing 15% heat-inactivated fetal bovine serum (Sigma Aldrich, F4135) and 1% penicillin-streptomycin-amphotericin-B mixture (Lonza, # 17–602E).

Protocol tips
Eagle's Minimum Essential Medium (EMEM, Quality Biological Inc., # 112-018-101) containing 15% heat-inactivated fetal bovine serum (Sigma Aldrich, F4135) and 1% penicillin-streptomycin-amphotericin-B mixture (Lonza, # 17–602E).

Publication protocol 1 Relevant paper

DMEM–Dulbecco's Modified Eagle Medium

Thermo Fisher Scientific

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	Dulbecco's Modified Eagle Medium (Life Technologies; UK) containing 10% foetal bovine serum (FBS), 0.2% amphotericin B (2 µg/ml), and 1% penicillin (100 U/ml) streptomycin (100 µg/ml) at 37°C in 5% CO2.

Protocol tips
Dulbecco's Modified Eagle Medium (Life Technologies; UK) containing 10% foetal bovine serum (FBS), 0.2% amphotericin B (2 µg/ml), and 1% penicillin (100 U/ml) streptomycin (100 µg/ml) at 37°C in 5% CO2.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM (Dulbecco's Modified Eagle's medium)

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Canine histiocytic cell line DH82 (ATCC CRL-10389) obtained from Public Health England was used between passage number 11 and 19.

Protocol tips
Canine histiocytic cell line DH82 (ATCC CRL-10389) obtained from Public Health England was used between passage number 11 and 19.

Manufacturer protocol Publication protocol 1 Relevant paper

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