Mammalian cell culture media H1299

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

HyClone RPMI 1640 media: Liquid

Cytiva

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd., Hangzhou, China), 100 U/ml penicillin and 100 µg/ml streptomycin (Beyotime Institute of Biotechnology, Shanghai, China)

Protocol tips
supplemented with 10% fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd., Hangzhou, China), 100 U/ml penicillin and 100 µg/ml streptomycin (Beyotime Institute of Biotechnology, Shanghai, China)

Publication protocol 1 Relevant paper

Gibco™ RPMI 1640 Medium

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	RPMI-1640 standard culture medium containing 10% FBS (v/v), and cultured at 37°C in a humidified atmosphere consisting of 5% CO2 and 95% air.

Protocol tips
RPMI-1640 standard culture medium containing 10% FBS (v/v), and cultured at 37°C in a humidified atmosphere consisting of 5% CO2 and 95% air.

Manufacturer protocol Publication protocol 1 Relevant paper

DMEM - Dulbecco's Modified Eagle Medium

Labtech

Upstream tips	Protocol tips	Downstream tips
	Supplement the cell with 10% fetal bovine serum (Biosera), 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco, Nuaille, France), and 2 mM L-glutamine.

Protocol tips
Supplement the cell with 10% fetal bovine serum (Biosera), 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco, Nuaille, France), and 2 mM L-glutamine.

Publication protocol 1 Relevant paper

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