Mammalian cell culture media HAOECv

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Gibco™ DMEM, high glucose

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum. L-glutamine (2 mmol/l and antibiotics (1% penicillin/streptomycin). Cells were cultured in a cell incubator providing a humidified environment, with 5% CO2 and 95% air at 37°C.

Protocol tips
supplemented with 10% fetal bovine serum. L-glutamine (2 mmol/l and antibiotics (1% penicillin/streptomycin). Cells were cultured in a cell incubator providing a humidified environment, with 5% CO2 and 95% air at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Growth Medium MV 2

PromoCell

Upstream tips	Protocol tips	Downstream tips
	supplemented with 5% foetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin

Protocol tips
supplemented with 5% foetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin

Manufacturer protocol Publication protocol 1 Relevant paper

Medium 200

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
supplement the media with low-serum.

Upstream tips
supplement the media with low-serum.

Manufacturer protocol Publication protocol 1 Relevant paper

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