Mammalian cell culture media HDF

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Fibroblasts growth media 2

PromoCell

Upstream tips	Protocol tips	Downstream tips
	NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Protocol tips
NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Protocol tips

NHDF were maintained in fibroblasts growth media 2 (FGM2, Promocell) under the physiological culture conditions (37°C, 5% CO2), and subcultured using DetachKit2-Promocell HEPES BSS (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffered saline solution); 0.04% trypsin/0.03% Ethylenediaminetetraacetic acid (EDTA); trypsin neutralizing solution (TNS) containing 0.05% trypsin inhibitor from soybean/0.1% bovine serum albumin).

Manufacturer protocol Publication protocol 1 Relevant paper

Dulbecco’s Modified Eagle’s Medium - high glucose

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Dermal fibroblasts were cultured in Dulbecco’s modified Eagle medium(DMEM; D6546, Sigma-Aldrich) supplemented with 10% foetal bovine serum (HyClone), 2 mM L-glutamine (G7513, Sigma-Aldrich)and 1% penicillin-streptomycin (P0781, Sigma-Aldrich) in a humidified incubator (37°C, 5% CO2). Cells were passaged once a week at a 1:4 split ratio

Protocol tips
Dermal fibroblasts were cultured in Dulbecco’s modified Eagle medium(DMEM; D6546, Sigma-Aldrich) supplemented with 10% foetal bovine serum (HyClone), 2 mM L-glutamine (G7513, Sigma-Aldrich)and 1% penicillin-streptomycin (P0781, Sigma-Aldrich) in a humidified incubator (37°C, 5% CO2). Cells were passaged once a week at a 1:4 split ratio

Manufacturer protocol Publication protocol 1 Relevant paper

Minimum Essential Medium (MEM)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% SERA Plus specially processed fetal bovine serum (FBS) (PAN biotech GmbH), penicillin (100 U/mL), streptomycin (100 μg/mL), and L-glutamine (2 mM).

Protocol tips
supplemented with 10% SERA Plus specially processed fetal bovine serum (FBS) (PAN biotech GmbH), penicillin (100 U/mL), streptomycin (100 μg/mL), and L-glutamine (2 mM).

Manufacturer protocol Publication protocol 1 Relevant paper

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