Mammalian cell culture media HepG2

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Dulbecco’s Modified Eagle’s Medium (DMEM)

Welgene

Upstream tips	Protocol tips	Downstream tips
	supplemented with 1% sodium pyruvate (Sigma), 1.5 g/L sodium bicarbonate, 10% fetal bovine serum and 1% antibiotics, including 1% penicillin/streptomycin and 25 ug/ml amphotericin B (Sigma) at 37 °C in 5% CO2

Protocol tips
supplemented with 1% sodium pyruvate (Sigma), 1.5 g/L sodium bicarbonate, 10% fetal bovine serum and 1% antibiotics, including 1% penicillin/streptomycin and 25 ug/ml amphotericin B (Sigma) at 37 °C in 5% CO2

Manufacturer protocol Publication protocol 1 Relevant paper

RPMI 1640

Biochrom

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin and 0.1 mg/ml streptomycin (both Sigma-Aldrich; Merck KGaA, Darmstadt, Germany)

Protocol tips
supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin and 0.1 mg/ml streptomycin (both Sigma-Aldrich; Merck KGaA, Darmstadt, Germany)

Manufacturer protocol Publication protocol 1 Relevant paper

RPMI 1640, 1X

WISENT BIOPRODUCTS

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS) (Welgene, Daegu, Korea) and 1% antibiotics

Protocol tips
supplemented with 10% fetal bovine serum (FBS) (Welgene, Daegu, Korea) and 1% antibiotics

Manufacturer protocol Publication protocol 1 Relevant paper

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