Mammalian cell culture media K562

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

HyClone RPMI 1640 media: Liquid

Cytiva

Upstream tips	Protocol tips	Downstream tips
	RPMI-1640 culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin and were continuously cultured in an incubator with saturated humidity (37°C and 5% CO2)

Protocol tips
RPMI-1640 culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 U/ml streptomycin and were continuously cultured in an incubator with saturated humidity (37°C and 5% CO2)

Publication protocol 1 Relevant paper

RPMI-1640 Medium

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS, Gibco, UK), 100 U/ml streptomycin, 100 U/ml penicillin, and 0.2 mmol/L-glutamine (Gibco, UK) at 37˚C in a humidified incubator with a 5% CO2 atmosphere

Protocol tips
supplemented with 10% fetal bovine serum (FBS, Gibco, UK), 100 U/ml streptomycin, 100 U/ml penicillin, and 0.2 mmol/L-glutamine (Gibco, UK) at 37˚C in a humidified incubator with a 5% CO2 atmosphere

Manufacturer protocol Publication protocol 1 Relevant paper

Gibco™ RPMI 1640 Medium

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	DMEM containing 10% FBS

Protocol tips
DMEM containing 10% FBS

Manufacturer protocol Publication protocol 1 Relevant paper

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