Mammalian cell culture media PCAEC

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

Gibco™ DMEM, high glucose

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	PAECs and the explant pieces of smooth muscle were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml), streptomycin (100 U/ml), 5 mM L-glutamine and 1.5 g/l sodium bicarbonate. The medium was changed every 2 days. The cells were cultured in an incubator at 37ºC and maintained in a humidified atmosphere with 5% CO2.

Protocol tips
PAECs and the explant pieces of smooth muscle were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml), streptomycin (100 U/ml), 5 mM L-glutamine and 1.5 g/l sodium bicarbonate. The medium was changed every 2 days. The cells were cultured in an incubator at 37ºC and maintained in a humidified atmosphere with 5% CO2.

Manufacturer protocol Publication protocol 1 Relevant paper

MCDB 131 Medium

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplement the medium with 15% fetal calf serum, penicillin (100 U/ml), streptomycin (100 U/ml), fungizone (250 μg/ml), and glutamine (2 mM).

Protocol tips
supplement the medium with 15% fetal calf serum, penicillin (100 U/ml), streptomycin (100 U/ml), fungizone (250 μg/ml), and glutamine (2 mM).

Manufacturer protocol Publication protocol 1 Relevant paper

Porcine Endothelial Cell Growth Medium

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Incubated at 37°C in a humidified atmosphere of 95% air and 5% CO2

Protocol tips
Incubated at 37°C in a humidified atmosphere of 95% air and 5% CO2

Publication protocol 1 Relevant paper

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