Mammalian cell culture media U937

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

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Found 3 matching solutions for this experiment

RPMI-1640 Medium

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Cell culture reagents and final concentrations used for activation of human (U937 and MDMs) and canine cells (DH82 and MDMs); * these reagents were used in control experiments to check for a potential increase of surface protein expression (specifically mentioned in the text) and are not part of routinely used protocol in presented experiments. DMEM, Dulbecco's Modified Eagle's medium; RPMI, Roswell Park Memorial Institute; v/v, volume per volume.

Protocol tips
Cell culture reagents and final concentrations used for activation of human (U937 and MDMs) and canine cells (DH82 and MDMs); * these reagents were used in control experiments to check for a potential increase of surface protein expression (specifically mentioned in the text) and are not part of routinely used protocol in presented experiments. DMEM, Dulbecco's Modified Eagle's medium; RPMI, Roswell Park Memorial Institute; v/v, volume per volume.

Protocol tips

Cell culture reagents and final concentrations used for activation of human (U937 and MDMs) and canine cells (DH82 and MDMs); * these reagents were used in control experiments to check for a potential increase of surface protein expression (specifically mentioned in the text) and are not part of routinely used protocol in presented experiments. DMEM, Dulbecco's Modified Eagle's medium; RPMI, Roswell Park Memorial Institute; v/v, volume per volume.

Manufacturer protocol Publication protocol 1 Relevant paper

HyClone RPMI 1640 media: Liquid

Cytiva

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS; HyClone) and 100 units penicillin, 0.1 mg streptomycin and 0.25 μg amphotericin B per ml

Protocol tips
supplemented with 10% fetal bovine serum (FBS; HyClone) and 100 units penicillin, 0.1 mg streptomycin and 0.25 μg amphotericin B per ml

Publication protocol 1 Relevant paper

IMDM

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Protocol tips
supplemented with 10% fetal bovine serum (FBS, PAA) and 10 U/mL penicillin-streptomycin (Gibco)

Manufacturer protocol Publication protocol 1 Relevant paper

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