Necrosis MCF7

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 4 matching solutions for this experiment

Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Abcam

Upstream tips	Protocol tips	Downstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells	• The Kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells with a flow cytometer or fluorescence microscope. In apoptotic cells, PS is transferred to the outer side of the plasma membrane and can be detected by its sensor, Apopxin Green (green fluorescence). The 7-aminoactinomycin D (red fluorescence), a membrane-impermeable dye, will label the nucleus of damaged cells, including both late apoptotic and necrotic cells. Live cells can be detected as blue fluorescence by CytoCalcein Violet 450 staining.

Upstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells
Protocol tips
• The Kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells with a flow cytometer or fluorescence microscope. In apoptotic cells, PS is transferred to the outer side of the plasma membrane and can be detected by its sensor, Apopxin Green (green fluorescence). The 7-aminoactinomycin D (red fluorescence), a membrane-impermeable dye, will label the nucleus of damaged cells, including both late apoptotic and necrotic cells. Live cells can be detected as blue fluorescence by CytoCalcein Violet 450 staining.

Manufacturer protocol 1 Relevant paper

Annexin V-FITC Kit, 200 Tests, RUO

Beckman Coulter

Upstream tips	Protocol tips	Downstream tips
• Annexin V kits employ recombinant annexin V labeled with biotin, fluorescein or R-phycoerythrin together with the viability dyes propidium iodide (PI) or 7-amino actinomycin D (7-AAD).	• Seed 5 × 10^3 cells/ well for melanoma cells and for breast cancer cells seed 3 × 10^3 cells/well in 96 well plates and permitted to adhere for 24 h at 37 °C in 5% CO2.

Upstream tips
• Annexin V kits employ recombinant annexin V labeled with biotin, fluorescein or R-phycoerythrin together with the viability dyes propidium iodide (PI) or 7-amino actinomycin D (7-AAD).
Protocol tips
• Seed 5 × 10^3 cells/ well for melanoma cells and for breast cancer cells seed 3 × 10^3 cells/well in 96 well plates and permitted to adhere for 24 h at 37 °C in 5% CO2.

Manufacturer protocol Publication protocol 1 Relevant paper

TACS Annexin V-FITC Apoptosis Detection Kit

R&D Systems

Upstream tips	Protocol tips	Downstream tips
• TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide	• Seed 5×10^4/cells per well

Upstream tips
• TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide
Protocol tips
• Seed 5×10^4/cells per well

Manufacturer protocol Publication protocol 1 Relevant paper

GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.

Upstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.

Manufacturer protocol Publication protocol 1 Relevant paper

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