Necrosis MCF7

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 4 matching solutions for this experiment

Upstream tips
• Assay type: Cell-based
• Platform: Flow cytometer, Fluorescence microscope
• Assay time: 1 hr
• Sample type: Adherent cells, Suspension cells
Protocol tips
• The Kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells with a flow cytometer or fluorescence microscope. In apoptotic cells, PS is transferred to the outer side of the plasma membrane and can be detected by its sensor, Apopxin Green (green fluorescence). The 7-aminoactinomycin D (red fluorescence), a membrane-impermeable dye, will label the nucleus of damaged cells, including both late apoptotic and necrotic cells. Live cells can be detected as blue fluorescence by CytoCalcein Violet 450 staining.
Upstream tips
• Annexin V kits employ recombinant annexin V labeled with biotin, fluorescein or R-phycoerythrin together with the viability dyes propidium iodide (PI) or 7-amino actinomycin D (7-AAD).
Protocol tips
• Seed 5 × 10^3 cells/ well for melanoma cells and for breast cancer cells seed 3 × 10^3 cells/well in 96 well plates and permitted to adhere for 24 h at 37 °C in 5% CO2.
Upstream tips
• TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide
Protocol tips
• Seed 5×10^4/cells per well
Upstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms