Necrosis MDA-MB-231

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

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Found 2 matching solutions for this experiment

GFP-CERTIFIED® Apoptosis/Necrosis detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.	• Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color.

Upstream tips
• This kit contained 7-amino-actinomycin D (7-AAD) and Annexin V, which were used to detect necroptosis and apoptosis, respectively.
Protocol tips
• Cells were observed under a confocal microscope (×200) after staining with 7-aminoactinomycin D (7-AAD) to identify necroptotic cells coated in red color.

Manufacturer protocol Publication protocol 1 Relevant paper

Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Abcam

Upstream tips	Protocol tips	Downstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells	• After removing the medium, the cells were washed three times using PBS.

Upstream tips
• Assay type: Cell-based • Platform: Flow cytometer, Fluorescence microscope • Assay time: 1 hr • Sample type: Adherent cells, Suspension cells
Protocol tips
• After removing the medium, the cells were washed three times using PBS.

Manufacturer protocol 1 Relevant paper

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