Found 1 discussion for this experiment
2 years ago
2 years ago by Hollie Fowler
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
Found 3 matching solutions for this experiment
|- Cell cultures centrifuged at 14,000 rpm for 5 min.
- Supernatants were passed through an 0.2-μm Acrodisc syringe filter.
|- Cell pellets are resuspended in 100 μl of B-PER protein extraction reagent and incubated for 10 min at room temperature. After, they are centrifuged at 14,000 rpm for 10 min, and supernatants are recovered.
- If there are insoluble precipitates (from membrane fractions) they can be resuspended with 50 μl of B-PER protein extraction reagent.