Protein Ladder IEF and 2-D Standards

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

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Found 2 matching solutions for this experiment

IEF Marker 3-10, Liquid Mix

SERVA Electrophoresis

Upstream tips	Protocol tips	Downstream tips
• IEF Marker 3-10 contains nine native proteins in the PI range 3.5 to 10.7 (13 isoforms).	• Staining with SERVA Violet 17 (cat. no. 35072 and 35074), SERVA Blue G (cat. no. 35050), SERVA Blue R (cat. no. 35051): 5 µl/lane for horizontal gels, 20 µl/lane for vertical gels. • Silver staining: 1:10 dilution and 5 µl or 20 µl/lane

Upstream tips
• IEF Marker 3-10 contains nine native proteins in the PI range 3.5 to 10.7 (13 isoforms).
Protocol tips
• Staining with SERVA Violet 17 (cat. no. 35072 and 35074), SERVA Blue G (cat. no. 35050), SERVA Blue R (cat. no. 35051): 5 µl/lane for horizontal gels, 20 µl/lane for vertical gels. • Silver staining: 1:10 dilution and 5 µl or 20 µl/lane

Manufacturer protocol

IEF and 2-D Protein Standards

Bio-Rad Laboratories

Upstream tips	Protocol tips	Downstream tips
• MW size range: Varies by standard (Ideal ten-band range from 10 kD to 250 kD). • IEF protein standards are a mixture of nine native proteins with pI ranging from 4.45 to 9.6.	• These standards are provided in a stable aqueous solution, and need no reconstitution or dilution prior to use.

Upstream tips
• MW size range: Varies by standard (Ideal ten-band range from 10 kD to 250 kD). • IEF protein standards are a mixture of nine native proteins with pI ranging from 4.45 to 9.6.
Protocol tips
• These standards are provided in a stable aqueous solution, and need no reconstitution or dilution prior to use.

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