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Found 3 matching solutions for this experiment
|Add Fixing Buffer to the dish and incubate for 10-15 minutes at room temperature.
Add 1X X-Gal staining solution and incubate the cells between 1-18 hours at 37°C in a humidified incubator.
|Remove medium and rinse cells in 1X PBS.
Add 1X lysis reagent.
Mix 20µl of cell lysate with 100µl of Luciferase Assay Reagent and measure the light produced.
|Seed ~ 2 × 105 cells/well
|Add diluted Galacton-Plus substrate 1:100 with Reaction Buffer Diluent.
Add 70µL of Reaction Buffer, mix, and incubate for 30-60 min
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