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Found 3 matching solutions for this experiment
|Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
|Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently.
Incubate at room temperature (20–25°C) for 60 min
|Seed 3 × 10^5 cells
|After 24 hours of transfection, perform assay and incubate for 30 min at 37 °C or until a faint yellow colour has developed.
|Read the absorbance at 420nm
|Treat cells with kit after 96 hrs of incubation.
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
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