Reporter gene assay β-galactosidase substrates - HepG2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 4 matching solutions for this experiment

Protocol tips
Fix cells in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS

Add 1 ml of the Staining Mixture per well and incubate for 16 h at 37 °C without CO2 until the cells are
stained blue
Protocol tips
Add 150µl of Assay 2X Buffer to sample.

Incubate the reactions at 37°C for 24 hours
Downstream tips
Read the absorbance at 420nm
Protocol tips
Fix cells with with 2% formaldehyde/0.2% glutaraldehyde.

Incubate for 12 hours at 37 ° C without Co2
Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution

Incubate overnight at 37 ° C without Co2
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