Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
Seed 1.5 × 105 cells/well in 6-well plate.	Fix cells with Fixative Solution for 15 min at room temperature. Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Upstream tips
Seed 1.5 × 105 cells/well in 6-well plate.
Protocol tips
Fix cells with Fixative Solution for 15 min at room temperature. Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Manufacturer protocol Publication protocol 1 Relevant paper

β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Promega

Upstream tips	Protocol tips	Downstream tips
	Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed	Read the absorbance at 420nm.

Protocol tips
Add 150µl of Assay 2X Buffer to sample. Incubate the reactions at 37°C for 30 minutes or until a faint yellow color has developed
Downstream tips
Read the absorbance at 420nm.

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence Cells Histochemical Staining Kit

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue

Protocol tips
Add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue

Manufacturer protocol Publication protocol 1 Relevant paper

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