Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Senescence Detection Kit - Merck

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
Seed 1.5×10^5 cells/well	Pretreat with 4 µM PAB for 3 days. Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.

Upstream tips
Seed 1.5×10^5 cells/well
Protocol tips
Pretreat with 4 µM PAB for 3 days. Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Add diluted Galacton-Star® substrate 1:50 with Reaction Buffer Diluent. Add 100 µL of Reaction Buffer, mix, and incubate for 30-90 min until light emission is maxima	Measure light signal in a luminometer for 0.1-1 sec/well.

Protocol tips
Add diluted Galacton-Star® substrate 1:50 with Reaction Buffer Diluent. Add 100 µL of Reaction Buffer, mix, and incubate for 30-90 min until light emission is maxima
Downstream tips
Measure light signal in a luminometer for 0.1-1 sec/well.

Manufacturer protocol Publication protocol 1 Relevant paper

Senescence β-Galactosidase Staining Kit - Cell Signaling

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
	Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Manufacturer protocol Publication protocol 1 Relevant paper

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