No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 3 matching solutions for this experiment
|Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
|Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently.
Incubate at room temperature (20–25°C) for 60 min
|Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature
Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.
|Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product