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Found 2 matching solutions for this experiment
|Pretreat cells with TM5441 for 24 h followed by Doxorubicin (50 nM) treatment in triplicate for 4 days.
|Fix cells with fixative solution for 15 min at room temperature.
Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.
|Fix cells for 5 minutes in 3.7% formaldehyde..
Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)
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