Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Found 3 matching solutions for this experiment

Protocol tips
Homogenize with reporter lysis buffer and centrifuged for 10 min at 12,000 g.

Add β-Glo assay solution and incubate for 1 h at room temperature.
Upstream tips
Seed 4×10^4 cells
Protocol tips
After 48h , fix cells for 10 min

Add β-Galactosidase Reporter Gene Stain and incubate for 12 h at 37°C.
Downstream tips
Read OD at 420 nm
Protocol tips
Add 10 µL of 200X SA-ß-Gal Substrate Solution and incubate at 37ºC for 4 hrs to overnight.
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