Reporter gene assay β-galactosidase substrates - yeast, Yarrowia lipolytica

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

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Protocol tips
Dilute Gal Screen substrate with Gal Screen Buffer.

Add to samples and incubate at 26-28C for 60-90 min
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