Found 1 discussion for this experiment
2 years ago
2 years ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 3 matching solutions for this experiment
|- The maximum recommended input of cells is 3 x 106.|
|- Traces of salt from the binding step may remain in the
sample if the column is not washed three times with Wash
Solution A. Salt may interfere with downstream
applications, and thus must be washed from the column.
- Ensure that 90 mL of 96 - 100% ethanol is added to the supplied Wash Solution A prior to use for poor RNA recovery.
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.