Found 1 discussion for this experiment
1 year ago
1 year ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 4 matching solutions for this experiment
|- To promote dissociation of nucleoprotein complexes, place the sample at room temperature (15–25°C) for 5 min after homogenization.|
|- Recommends for a cleanup of the redissolved RNA using RNeasy® Kits, which are based on silica-membrane technology, in order to remove any contaminating phenol.
- If A260/A280 is low or if DNA containation is high, reduce the amount of starting material and/or increase the volume of QIAzol Lysis Reagent and the homogenization time.
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.