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2 years ago
2 years ago by Ralf Friedmann
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Found 4 matching solutions for this experiment
|- This kit is designed for small amounts of cells (max 10^5).|
|- For RNA to dissolve better and to yeild high level preheat elution buffer at 60C.|
|- Because this kit is designed for low amounts of starting material, the total RNA yield can be low and might be hard to measure using a Nanodrop. Use a fluorescence-based system to quantify.|
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.