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Found 4 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
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- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).
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Protocol tips |
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer). |
Downstream tips |
- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).
|
Upstream tips |
Protocol tips |
Downstream tips |
- Aliquot rDNase and store at -20 °C.
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- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.
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Upstream tips |
- Aliquot rDNase and store at -20 °C.
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Protocol tips |
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.
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Upstream tips |
Protocol tips |
Downstream tips |
- Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results. |
- An additional wash with 75% ethanol improves 260/280 ratio. |
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Upstream tips |
- Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results. |
Protocol tips |
- An additional wash with 75% ethanol improves 260/280 ratio. |
Upstream tips |
Protocol tips |
Downstream tips |
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- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
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Protocol tips |
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
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