No discussions found
Found 3 matching solutions for this experiment
|C. neoformans might require extra effort for lysis (bead beating, see reference)
To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
|"- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C"
|For lysis of yeast, extra enzymatic or mechanical disruption is recommended.|
|Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required|
|DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit|