RNA isolation / purification Yeast - Schizophyllum commune

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

Protocol tips
RNA of seven days old, solid cultures of the strains 12–43, E6 and ∆dhc2 was isolated using RNeasy Plant Mini Kit performing an additional DNAse digestion with RNase-Free DNase Set (both Qiagen, Hilden,Germany). RNA-sequencing was performed by LGC Genomics, Berlin (Germany) with mRNA-based cDNA-libraries constructed from sequencing adaptors ligated to cDNA fragments

TRIzol Reagent + NucleoSpin® RNA

TRIzol Reagent

Thermo Fisher Scientific

NucleoSpin® RNA

Macherey Nagel

Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms