ROS assay cell type - DU145

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

ROS-ID® Total ROS/Superoxide detection kit

Enzo Life Sciences

Upstream tips	Protocol tips	Downstream tips
	- After 24h of cell seeding, cells were treated with ROS detection solution and incubate under normal culture conditions for 1 h. - After washing cells off from reagent, cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidant

Protocol tips
- After 24h of cell seeding, cells were treated with ROS detection solution and incubate under normal culture conditions for 1 h. - After washing cells off from reagent, cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidant

Manufacturer protocol Publication protocol 1 Relevant paper

OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	- Cells were incubated with DCFH-DA for 1 h at 37°C. - After incubation with DCFH-DA cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C

Protocol tips
- Cells were incubated with DCFH-DA for 1 h at 37°C. - After incubation with DCFH-DA cells were treated with Tanshinone I and/or TRAIL for 1 h at 37°C

Manufacturer protocol Publication protocol 1 Relevant paper

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