ROS assay cell type - HEK293

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- 0, 500, 1000, 5000, and 1000 wild type and autobioluminescent HEK293 cells were platted
Protocol tips
- Cells were incubated at 37°C and 5% CO2 for 18 h before the addition of 20 µl 125 µM H2O2 substrate.

- each well was then incubated for an additional 6 h followed by bioluminescent measurements of H2O2 levels.
Protocol tips
- Cells were treated with 0.33 mm H2O2
Downstream tips
- ROS levels were measured after 1 and 4 h of treatment.
Protocol tips
- Cells were treated with superoxide Detection Solution and then incubated for 30 min
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