ROS assay cell type - Huh7

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 1 matching solution for this experiment

ROS-Glo™ H2O2 Assay

Promega

Upstream tips	Protocol tips	Downstream tips
- Cells were pretreated with lipid and/or doxorubicin	- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation

Upstream tips
- Cells were pretreated with lipid and/or doxorubicin
Protocol tips
- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation

Manufacturer protocol Publication protocol 1 Relevant paper

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