ROS assay cell type - Huh7

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Upstream tips
- Cells were pretreated with lipid and/or doxorubicin
Protocol tips
- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation
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