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Found 4 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
- 1-1.5×10^5 cells were platted.
- Cells were pre-treated with increasing concentrations of NS1 for 30 min at 37°C |
- CellROX Reagent at a final concentration of 2 μM and incubated for 30 min. |
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| Upstream tips |
- 1-1.5×10^5 cells were platted.
- Cells were pre-treated with increasing concentrations of NS1 for 30 min at 37°C |
| Protocol tips |
| - CellROX Reagent at a final concentration of 2 μM and incubated for 30 min. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - Cells were cultured in 96 well plate |
- Cells were treated with fluorogenic probe 2′,7′-dichlorodihydrofluorescin diacetate and incubated for 1h |
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| Upstream tips |
| - Cells were cultured in 96 well plate |
| Protocol tips |
| - Cells were treated with fluorogenic probe 2′,7′-dichlorodihydrofluorescin diacetate and incubated for 1h |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were incubated for 1h after adding fluorogenic probe.
- The assay was terminated by adding cell lysis buffer and incubating for 5 min. |
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| Protocol tips |
- Cells were incubated for 1h after adding fluorogenic probe.
- The assay was terminated by adding cell lysis buffer and incubating for 5 min. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were treated with carboxy-H2DCFDA at a final concentration of 25μM in darkness at 37°C for 30 min. |
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| Protocol tips |
| - Cells were treated with carboxy-H2DCFDA at a final concentration of 25μM in darkness at 37°C for 30 min. |
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