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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were treated with 5 μM MitoSOX Red for 30 min at room temperature,
- Cells were exposed to the β-adrenergic agonist (ISO; 100 nM) and assessed by confocal images. |
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| Protocol tips |
- Cells were treated with 5 μM MitoSOX Red for 30 min at room temperature,
- Cells were exposed to the β-adrenergic agonist (ISO; 100 nM) and assessed by confocal images. |
| Upstream tips |
Protocol tips |
Downstream tips |
| - 0.5 × 105 cells were platted in a 96 well plate. |
- 20 μl ROS detection reagent stock solution and 10 ml assay buffer and incubated at 5% CO2, 37°C incubator for 1 hr. |
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| Upstream tips |
| - 0.5 × 105 cells were platted in a 96 well plate. |
| Protocol tips |
| - 20 μl ROS detection reagent stock solution and 10 ml assay buffer and incubated at 5% CO2, 37°C incubator for 1 hr. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C. |
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| Protocol tips |
| - Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C. |
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