ROS assay cell type - mouse cardiomyocytes

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells were treated with 5 μM MitoSOX Red for 30 min at room temperature,

- Cells were exposed to the β-adrenergic agonist (ISO; 100 nM) and assessed by confocal images.
Upstream tips
- 0.5 × 105 cells were platted in a 96 well plate.
Protocol tips
- 20 μl ROS detection reagent stock solution and 10 ml assay buffer and incubated at 5% CO2, 37°C incubator for 1 hr.
Protocol tips
- Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C.
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