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Found 4 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
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- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent
DCF-DiOxy and DCFH solutions must be protected from light. |
| Downstream tips |
- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent
DCF-DiOxy and DCFH solutions must be protected from light. |
| Upstream tips |
Protocol tips |
Downstream tips |
- Cells were pre-treated with different concentrations of Nx.
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- Cells were incubated with 5 μm CellROX Deep Red reagent for 30 min at 37°C |
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| Upstream tips |
- Cells were pre-treated with different concentrations of Nx.
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| Protocol tips |
| - Cells were incubated with 5 μm CellROX Deep Red reagent for 30 min at 37°C |
| Upstream tips |
Protocol tips |
Downstream tips |
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- H2O2 substrate was added together with SW43-DOX.
- After 6h, detection solution was added and luminescence was measured 20 min. |
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| Protocol tips |
- H2O2 substrate was added together with SW43-DOX.
- After 6h, detection solution was added and luminescence was measured 20 min. |
| Upstream tips |
Protocol tips |
Downstream tips |
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- After wash step, cells were stained with ROS detection solution and incubated at 30 min, 37˚C |
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| Protocol tips |
| - After wash step, cells were stained with ROS detection solution and incubated at 30 min, 37˚C |
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