ROS assay cell type - PANC-, BxPC-3 human pancreas

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 4 matching solutions for this experiment

Downstream tips
- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent
DCF-DiOxy and DCFH solutions must be protected from light.
Upstream tips
- Cells were pre-treated with different concentrations of Nx.
Protocol tips
- Cells were incubated with 5 μm CellROX Deep Red reagent for 30 min at 37°C
Protocol tips
- H2O2 substrate was added together with SW43-DOX.

- After 6h, detection solution was added and luminescence was measured 20 min.
Protocol tips
- After wash step, cells were stained with ROS detection solution and incubated at 30 min, 37˚C
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