ROS assay cell type - PC12

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded for 24h and then treated with 1X DCFH-DA and incubated for 60 min at 37 °C	- Cells were treated with Ramizol™ at 0.1 mg mL−1, A-T (10 μM) and stressors 1 mM H2O2, 500 μM menadione and 100 mM glutamate for 60min

Upstream tips
- Cells were seeded for 24h and then treated with 1X DCFH-DA and incubated for 60 min at 37 °C
Protocol tips
- Cells were treated with Ramizol™ at 0.1 mg mL−1, A-T (10 μM) and stressors 1 mM H2O2, 500 μM menadione and 100 mM glutamate for 60min

Manufacturer protocol Publication protocol 1 Relevant paper

CM-H2DCFDA (General Oxidative Stress Indicator)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with 5 µM CM-H2DCFDA. - Cells were incubated at 37C for 20 min.

Protocol tips
- Cells were treated with 5 µM CM-H2DCFDA. - Cells were incubated at 37C for 20 min.

Manufacturer protocol Publication protocol 1 Relevant paper

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