ROS assay cell type - SH-SY5Y human neuroblastoma

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- Reconstitute ROS Detection Reagent with 40 mL of
DMSO to generate the 500´ ROS Detection Reagent Stock Solution.
Protocol tips
- The Master Reaction Mix is enough for one plate. The Master Reaction Mix is best used within 2 hours.
Protocol tips
- The cells were incubated with test compounds and H2O2 substrate solution for 6 h
Downstream tips
- ROS assay were read at 480/530 nm
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