ROS assay cell type - U87

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 2 matching solutions for this experiment

CellROX™ Deep Red Reagent, for oxidative stress detection

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with 5 μM CellROX® Deep Red Reagent. - Cells were incubated at 37 °C for 30 min

Protocol tips
- Cells were treated with 5 μM CellROX® Deep Red Reagent. - Cells were incubated at 37 °C for 30 min

Manufacturer protocol Publication protocol 1 Relevant paper

OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
- tissue were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min before the treatment.

Upstream tips
- tissue were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min before the treatment.

Manufacturer protocol Publication protocol 1 Relevant paper

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