ROS assay cell type - U87

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Protocol tips
- Cells were treated with 5 μM CellROX® Deep Red Reagent.

- Cells were incubated at 37 °C for 30 min
Upstream tips
- tissue were homogenized in cold PBS solution (20 mg/ml) and centrifuged at 10 000× g for 5 min before the treatment.
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