siRNA / miRNA gene silencing Human - BCBL-1 xCT

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

Dharmacon

Upstream tips	Protocol tips	Downstream tips
	Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 24 hours.

Protocol tips
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 24 hours.

Manufacturer protocol Publication protocol 1 Relevant paper

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