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|Seed 1.5–6 x 10^5 cells/weel in a 6 well plate.|
|siRNA final concentration - 50 nM
Incuabte cells for 24h and add diluted siRNA to HiPerFect Transfection Reagent to make a mixture
Incubate the mixture for 5–10 min at room temperature.
Add mixture to cells and incubate for 72 h