siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

siRNA Junction plakoglobin (SASI_Hs01_00246520) + Lipofectamine® 2000 Transfection Reagent

Upstream tips
Seed 200 000 cells in 6 well plate for 24 hours
Protocol tips
siRNA concentration-40nm

Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 1 day at 37°C and replace medium.

Incubate for another 48 hours.

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