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|Seed 30000 cells/well|
|The siRNA sequences (5′-to-3′) were:
1: GGCAAGUAAGUCCAUAUCATT (sense) and UGAUAUGGACUUGCCTC (antisense)
2: GGCUCACAGUAGUGGUAATT (sense) and UUACCACUACUGUGAGCCTT (antisense)
SKIL siRNA concentration- 50 nM
Add INTERFERin to siRNA duplexes and incubate for 10min at RT after homogenization.
Remove culture medium from cells and add incubate mixture.
Incubate the plate at 37°C for 24 to 72 h.