siRNA / miRNA gene silencing Human - KLM-1 RUNX3

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

siRNA RUNX3

Thermo Fisher Scientific

Qiagen

Upstream tips	Protocol tips	Downstream tips
	Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. Incubate cells with serum starved for 48 h.

Protocol tips
Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. Incubate cells with serum starved for 48 h.

Manufacturer protocol Publication protocol 1 Relevant paper

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